Skip to main content
Holiday Schedule: Addgene will be closed November 26th & 27th for the Thanksgiving Holiday. Order processing and shipping may be delayed during this week. For questions about your shipment please contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pEF1-alpha-V
(Plasmid #27290)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27290 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEF1/V5-HisA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    integrin alpha V
  • Alt name
    CD51
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3000
  • Mutation
    Isoform 2
  • GenBank ID
    EU332844
  • Entrez Gene
    ITGAV (a.k.a. CD51, MSK8, VNRA, VTNR)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site Not I (*note two sites are present) (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1-alpha-V was a gift from Timothy Springer (Addgene plasmid # 27290 ; http://n2t.net/addgene:27290 ; RRID:Addgene_27290)
  • For your References section:

    Global conformational rearrangements in integrin extracellular domains in outside-in and inside-out signaling. Takagi J, Petre BM, Walz T, Springer TA. Cell. 2002 Sep 6. 110(5):599-11. 10.1016/S0092-8674(02)00935-2 PubMed 12230977