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Integrin alpha5(1-623)-AHCys
(Plasmid #27301)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 27301 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    integrin alpha 5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    alpha 5(1-623)+GG+TEV+GGEN+AHCys
  • Entrez Gene
    ITGA5 (a.k.a. CD49e, FNRA, VLA-5, VLA5A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer EF1a-Fwd
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

There is a minor discrepancy between BLAST truncation position and depositor's truncation position. The discrepancies could be the secretion peptide that the depositing lab does not include in their numbering, or the C-terminal tags (that's about 40 aa.)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Integrin alpha5(1-623)-AHCys was a gift from Timothy Springer (Addgene plasmid # 27301 ; ; RRID:Addgene_27301)
  • For your References section:

    Structure of integrin alpha5beta1 in complex with fibronectin. Takagi J, Strokovich K, Springer TA, Walz T. EMBO J. 2003 Sep 15. 22(18):4607-15. 10.1093/emboj/cdg445 PubMed 12970173