FC858
(Plasmid #27328)

• Depositing Lab: Fred Cross
• Publication: Cross et al Yeast. 2010 Oct 22. ():.

Backbone

• Vector backbone: RS406
• Backbone size w/o insert (bp): 4384
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CDH1
• Alt name: RS406-CDH1-m11-HOcs
• Species: S. cerevisiae (budding yeast)
• Mutation: 11 Phosphorylation site point mutations: T12A S16A S42A T157A S169A T173A T176A S227A S239A S418A S436A
• Entrez Gene: CDH1 (a.k.a. YGL003C, HCT1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry