Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


pDR-GW FLIPglu-30μΔ13V
(Plasmid #27998)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 27998 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6866
  • Vector type
    Yeast Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    E. Coli
  • Insert Size (bp)
  • Mutation
    D154A in mglB
  • Tags / Fusion Proteins
    • eCFP (N terminal on insert)
    • eYFP (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site Gateway-based cloning (not destroyed)
  • 3′ cloning site Gateway-based cloning (not destroyed)
  • 5′ sequencing primer pPMA-For 5'-AACAATCGTTAATAATTAATTAATTGG
  • 3′ sequencing primer pHybLex_rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDR-GW FLIPglu-30μΔ13V was a gift from Wolf Frommer (Addgene plasmid # 27998 ; ; RRID:Addgene_27998)
  • For your References section:

    Dynamic analysis of cytosolic glucose and ATP levels in yeast using optical sensors. Bermejo C, Haerizadeh F, Takanaga H, Chermak D, Frommer WB. Biochem J. 2010 Dec 1. 432(2):399-406. 10.1042/BJ20100946 PubMed 20854260