Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is operating at a reduced capacity.

  • Pending order? We will email to confirm that your organization can accept shipments.
  • Conducting coronavirus or COVID-19 research? Email us at [email protected] with your order or deposit number so we can prioritize it.

Learn more about our current shipping status and COVID-19 resources.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pTGV-ProK Express SMR5454
(Plasmid #28034)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28034 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

pTGV-ProkExpress is pTGV-Light (Addgene plasmid number 28032) with an arabinose-inducible promoter (PBAD) upstream of the multiple cloning site to allow regulatable expression of the transgene. The AraC protein, required for PBAD regulation must be expressed from its normal chromosomal location.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTGV-ProK Express SMR5454 was a gift from Susan Rosenberg (Addgene plasmid # 28034 ; http://n2t.net/addgene:28034 ; RRID:Addgene_28034)
  • For your References section:

    The TGV transgenic vectors for single-copy gene expression from the Escherichia coli chromosome. Gumbiner-Russo LM, Lombardo MJ, Ponder RG, Rosenberg SM. Gene. 2001 Jul 25. 273(1):97-104. 10.1016/S0378-1119(01)00565-0 PubMed 11483365