GRM7
(Plasmid #28110)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Q2 (unpublished)

Backbone

• Vector backbone: pFHMSP-LIC-N (Addgene plasmid 26095)
• Backbone manufacturer: SGC
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GRM7:DCC014-D04:C211307
• Alt name: PDB: 3MQ4
• Alt name: metabotropic glutamate receptor 7, extracellular ligand binding domain
• Species: H. sapiens (human)
• Insert Size (bp): 1431
• Mutation: contains ligand binding domain, residues 37-513.
• GenBank ID: NM_000844
• Entrez Gene: GRM7 (a.k.a. GLUR7, GPRC1G, MGLU7, MGLUR7, PPP1R87)
• Tag / Fusion Protein:
  • His tag with TEV site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ligation-independent cloning (unknown if destroyed)
• 3′ cloning site: ligation-independent cloning (unknown if destroyed)
• 5′ sequencing primer: polyhedrin-fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 3MQ4. http://www.thesgc.org/structures/3MQ4/