Flag-TRIM24-F979A/N980A shRescue
(Plasmid #28143)

• Depositing Lab: Michelle Barton
• Publication: Barton Lab Unpublished Plasmids (unpublished)

Backbone

• Vector backbone: pCMX-(Flag)x2
• Backbone manufacturer: Barton Lab
• Backbone size w/o insert (bp): 4500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRIM24
• Species: H. sapiens (human)
• Insert Size (bp): 3200
• Mutation: A489V, F979A and N980A; aa 308 and 309 mutated for shRNA rescue
• Entrez Gene: TRIM24 (a.k.a. PTC6, RNF82, TF1A, TIF1, TIF1A, TIF1ALPHA, hTIF1)
• Tag / Fusion Protein:
  • FLAGX2 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: 5'-GTCACACCACAGAAGTAAGGT-3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified a A489V mutation present in TRIM24 sequence. This mutation was not intentional, but the depositing laboratory states that it does not affect plasmid function.