pTH353-SUP45wt
(Plasmid #29371)

• Depositing Lab: Tobias von der Haar
• Publication: Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):5479-92.

Backbone

• Vector backbone: pRS315
• Backbone size w/o insert (bp): 5976
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SUP45 gene encoding translation release factor 1 from S. cerevisiae
• Alt name: eRF1
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2984
• Mutation: Wild type gene including genomic sequence 1432 bp upstream of AUG and 241 bp downstream of stop codon.
• GenBank ID: NM_001178491
• Entrez Gene: SUP45 (a.k.a. YBR143C, SAL4, SUP1, SUP47)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: HinDiii (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry