pcDNA5FRT/FLAG-Ino80 ΔNC (267-1261)
(Plasmid
#29439)
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Depositing Labs
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Depositing OrganizationStowers Institute for Medical Research
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 29439 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 29439-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 29439-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA5-FRT
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5070
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameINO80
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Alt namepcDNA5FRT/FLAG-Ino80 dNC
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SpeciesH. sapiens (human)
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Insert Size (bp)2985
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MutationINO80 267-1261 (a.a.) with Q1116K mutation
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GenBank IDNP_060023
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Entrez GeneINO80 (a.k.a. INO80A, INOC1)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI / XhoI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This construct is exactly as described in J Biol Chem. 2011 Apr 1;286(13):11283-9, including the Q1116K mutation.
DNA (Catalog # 29439-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 29439-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5FRT/FLAG-Ino80 ΔNC (267-1261) was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 29439 ; http://n2t.net/addgene:29439 ; RRID:Addgene_29439) -
For your References section:
Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling. Chen L, Cai Y, Jin J, Florens L, Swanson SK, Washburn MP, Conaway JW, Conaway RC. J Biol Chem. 2011 Apr 1;286(13):11283-9. doi: 10.1074/jbc.M111.222505. Epub 2011 Feb 8. 10.1074/jbc.M111.222505 PubMed 21303910