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pMAX deltaN-ERG
(Plasmid #29447)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29447 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 29447-D50 50 μg of DNA in Tris buffer $495
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DNA 29447-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pMAX-DEST
  • Backbone manufacturer
    Amaxa
  • Backbone size w/o insert (bp) 2800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ERG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    Deletion of first 33 aa of ERG
  • Entrez Gene
    ERG (a.k.a. LMPHM14, erg-3, p55)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-Fwd
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PCR fragment amplified using IMAGE clone 6052140. Cloned into pCR8/GW/TOPO and then moved to pMAX-DEST using Gateway.

For transient expression of human deltaN-ERG. Has endogenous stop codon (no V5).

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAX deltaN-ERG was a gift from Valeri Vasioukhin (Addgene plasmid # 29447 ; http://n2t.net/addgene:29447 ; RRID:Addgene_29447)

  • For your References section:

    A causal role for ERG in neoplastic transformation of prostate epithelium. Klezovitch O, Risk M, Coleman I, Lucas JM, Null M, True LD, Nelson PS, Vasioukhin V. Proc Natl Acad Sci U S A. 2008 Feb 12. 105(6):2105-10. 10.1073/pnas.0711711105 PubMed 18245377
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