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pOSCAR
(Plasmid #29639)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 29639 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pPZP-RCS2
  • Backbone manufacturer
    Stanton Gelvin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    DB3.1 (ccdB resistant)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None
  • Alt name
    OSCAR
  • GenBank ID
    HM623914

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To constuct this vector the 2.5 kb Gateway cassette attP2R-ccdB-cmR-attP3 was cloned into pCR2.1-TOPO (Invitrogen) generating plasmid pTOPO-attP2R-ccdB-attP3. The P2R-ccdB-cmR-P3 cassette was then excised from pTOPO-attP2R-ccdB-attP3 by digestion with HindIII and KpnI and inserted in the binary vector pPZP-RCS2.

Note that Addgene's sequencing results contain a single nucleotide mismatch at bp#4262 when compared to the depositor's sequence. This discrepancy does not affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOSCAR was a gift from David_L Andrews & Scott Gold (Addgene plasmid # 29639 ; http://n2t.net/addgene:29639 ; RRID:Addgene_29639)
  • For your References section:

    One Step Construction of Agrobacterium-Recombination-ready-plasmids (OSCAR), an efficient and robust tool for ATMT based gene deletion construction in fungi. Paz Z, Garcia-Pedrajas MD, Andrews DL, Klosterman SJ, Baeza-Montanez L, Gold SE. Fungal Genet Biol. 2011 Mar 5. ():. 10.1016/j.fgb.2011.02.003 PubMed 21362493