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HL 3327 (=HL 716 + pHL 1009)
(Plasmid #30060)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 30060 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    ColE (from pZ system) + AmpR
  • Backbone manufacturer
    Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997)
  • Backbone size w/o insert (bp) 2000
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    HL 716
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pLlacO-1:MicC-mCherry, pLtetO-1:RBS (st7) gfp
  • Species
    E. coli
  • Insert Size (bp)
    2400
  • Tag / Fusion Protein
    • mCherry, GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AatII (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer ctcatgagcggatacat atttgaa
  • 3′ sequencing primer agctgatacc gctcgccgca gccgaacg
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HL 3327 (=HL 716 + pHL 1009) was a gift from Han Lim (Addgene plasmid # 30060 ; http://n2t.net/addgene:30060 ; RRID:Addgene_30060)
  • For your References section:

    Disruption of small RNA signaling caused by competition for Hfq. Hussein R, Lim HN. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. 10.1073/pnas.1010082108 PubMed 21189298