HL 3325 (=HL 770 + rhyB knockout_KanR)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||30065||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2000
Vector typeSynthetic Biology
Growth in Bacteria
Growth Strain(s)HL 770
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Same strain as HL 3325 except that is not cured of Kanamycin resistence at rhyB.
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HL 3325 (=HL 770 + rhyB knockout_KanR) was a gift from Han Lim (Addgene plasmid # 30065)
For your References section:Disruption of small RNA signaling caused by competition for Hfq. Hussein R, Lim HN. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. 10.1073/pnas.1010082108 PubMed 21189298