pSG5-FER
(Plasmid
#30191)
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Depositing Lab
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Depositing OrganizationChildren's Hospital Los Angeles
Located in United States of America -
Publication
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Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 30191 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 30191-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 30191-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepSG5
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4076
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFer tyrosine kinase
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Alt nameFER
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Alt nameFer (fps/fes related) tyrosine kinase
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SpeciesH. sapiens (human)
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Insert Size (bp)2950
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GenBank IDNM_005246
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Entrez GeneFER (a.k.a. PPP1R74, TYK3, p94-Fer)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Fer pSG5. Fer is a non-receptor tyrosine kinase located on human chromosome 5q21. Gene ID 2241; HPRD 01491; NM_005246.
The construct was put together by Joanne ten Hoeve in 1993 and contains the complete coding region of human Fer.
DNA (Catalog # 30191-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 30191-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG5-FER was a gift from Nora Heisterkamp (Addgene plasmid # 30191 ; http://n2t.net/addgene:30191 ; RRID:Addgene_30191) -
For your References section:
Isolation and sequence analysis of a novel human tyrosine kinase gene. Hao QL, Heisterkamp N, Groffen J. Mol Cell Biol. 1989 Apr . 9(4):1587-93. PubMed 2725517