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pTRE ZO1myc
(Plasmid #30318)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30318 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 30318-D50 50 μg of DNA in Tris buffer $495
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DNA 30318-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pTRE
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3100
  • Vector type
    Mammalian Expression
  • Selectable markers
    NONE

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zonula Occludens-1
  • Alt name
    TJP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5376
  • Entrez Gene
    TJP1 (a.k.a. ZO-1)
  • Tag / Fusion Protein
    • myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco RI (not destroyed)
  • 3′ cloning site Eco RI (not destroyed)
  • 5′ sequencing primer GACCTCCATAGAAGACACC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE ZO1myc was a gift from Alan Fanning (Addgene plasmid # 30318 ; http://n2t.net/addgene:30318 ; RRID:Addgene_30318)

  • For your References section:

    The unique-5 and -6 motifs of ZO-1 regulate tight junction strand localization and scaffolding properties. Fanning AS, Little BP, Rahner C, Utepbergenov D, Walther Z, Anderson JM. Mol Biol Cell. 2007 Mar . 18(3):721-31. 10.1091/mbc.e06-08-0764 PubMed 17182847
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