Magoh198delG
(Plasmid #30481)

• Depositing Labs: William Pavan, Debra Silver
• Publication: Silver et al Nat Neurosci. 2010 May . 13(5):551-8.

Backbone

• Vector backbone: pCMV-Sport6
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Magoh
• Alt name: Mago-nashi homolog
• Species: M. musculus (mouse)
• Insert Size (bp): 1000
• Mutation: 198delG (point nucleotide deletion)
• Entrez Gene: Magoh (a.k.a. Mago-m, Mos2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: Sp6
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Open Biosystems

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

AluI digestion generates 341 bp, 189 bp, 112 bp. Wt only generates 341 bp +300 bp fragments.