Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||30484||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationDeletion of the C-terminal Ubiquitin binding domain- aa1044-1273 (BUZ domain)
Entrez GeneHDAC6 (a.k.a. CPBHM, HD6, JM21, PPP1R90)
/ Fusion Protein
- FLAG (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA-HDAC6.ΔBuz-FLAG was a gift from Tso-Pang Yao (Addgene plasmid # 30484 ; http://n2t.net/addgene:30484 ; RRID:Addgene_30484)
For your References section:The deacetylase HDAC6 regulates aggresome formation and cell viability in response to misfolded protein stress. Kawaguchi Y, Kovacs JJ, McLaurin A, Vance JM, Ito A, Yao TP. Cell. 2003 Dec 12. 115(6):727-38. 10.1016/S0092-8674(03)00939-5 PubMed 14675537