Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||30495||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)3700
Entrez GeneAHI1 (a.k.a. AHI-1, JBTS3, ORF1, dJ71N10.1)
/ Fusion Protein
- DsRed2 (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site ApaI (unknown if destroyed)
- 5′ sequencing primer DsRed1-C (Common Sequencing Primers)
Total plasmid size: 8.4kb
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDsRed2-C1-Ahi1 was a gift from Joseph Gleeson (Addgene plasmid # 30495 ; http://n2t.net/addgene:30495 ; RRID:Addgene_30495)
For your References section:Impaired Wnt-beta-catenin signaling disrupts adult renal homeostasis and leads to cystic kidney ciliopathy. Lancaster MA, Louie CM, Silhavy JL, Sintasath L, Decambre M, Nigam SK, Willert K, Gleeson JG. Nat Med. 2009 Sep . 15(9):1046-54. 10.1038/nm.2010 PubMed 19718039