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AnkyrinB-2XHA
(Plasmid #31057)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31057 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 31057-D50 50 μg of DNA in Tris buffer $495
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DNA 31057-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    Modified pEGFP-N1 (see note)
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ankyrin B
  • Alt name
    ankyrin 2
  • Species
    H. sapiens (human)
  • Entrez Gene
    ANK2 (a.k.a. ANK-2, CFAP87, FAP87, LQT4, brank-2)
  • Tag / Fusion Protein
    • 2XHA (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HA-ankyrin-B was prepared based on an ankyrin-B-GFP template in a pEGFP-N1 vector (Mohler et al., 2002). GFP was cut out between the PmeI and the NotI sites and replaced with a 2XHA-tag encoding sequence (YPYDVPDYA) with a PmeI site on its 5′ end and a stop codon followed by a NotI site on the 3′ end that was synthesized by PCR.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AnkyrinB-2XHA was a gift from Vann Bennett (Addgene plasmid # 31057 ; http://n2t.net/addgene:31057 ; RRID:Addgene_31057)

  • For your References section:

    Ankyrin-B interactions with spectrin and dynactin-4 are required for dystrophin-based protection of skeletal muscle from exercise injury. Ayalon G, Hostettler JD, Hoffman J, Kizhatil K, Davis JQ, Bennett V. J Biol Chem. 2011 Mar 4. 286(9):7370-8. 10.1074/jbc.M110.187831 PubMed 21186323

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