|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31112||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameACE2 promoter
SpeciesH. sapiens (human)
Insert Size (bp)300
Mutationpromoter region -202 to +103
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
promoter luciferase reporter
Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ACE2(-202)-luc was a gift from Gerhart Ryffel (Addgene plasmid # 31112 ; http://n2t.net/addgene:31112 ; RRID:Addgene_31112)
For your References section:Identification of target genes of the transcription factor HNF1beta and HNF1alpha in a human embryonic kidney cell line. Senkel S, Lucas B, Klein-Hitpass L, Ryffel GU. Biochim Biophys Acta. 2005 Dec 20. 1731(3):179-90. 10.1016/j.bbaexp.2005.10.003 PubMed 16297991