|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31119||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3176
Vector typeMammalian Expression, RNAi
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameTatSF1 shRNA
SpeciesH. sapiens (human)
Entrez GeneHTATSF1 (a.k.a. TAT-SF1, TATSF1, dJ196E23.2)
- Cloning method Restriction Enzyme
- 5′ sequencing primer H1 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
shRNA TatSF1 Target: GAGTCAGATGACAAGGAAG
NB: This shRNA for TatSF1 and Plasmid #31120 both work very well.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSuper-TatSF1 AB was a gift from Mariano Garcia-Blanco (Addgene plasmid # 31119 ; http://n2t.net/addgene:31119 ; RRID:Addgene_31119)
For your References section:Tat-SF1 is not required for Tat transactivation but does regulate the relative levels of unspliced and spliced HIV-1 RNAs. Miller HB, Saunders KO, Tomaras GD, Garcia-Blanco MA. PLoS One. 2009 . 4(5):e5710. 10.1371/journal.pone.0005710 PubMed 19479034