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Depositing Lab
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Depositing OrganizationScripps California (The Scripps Research Institute)
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31156 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 31156-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 31156-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneBiCS2eGFP
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Backbone manufacturerDavid Turner (University of Michigan Medical Center)
- Backbone size w/o insert (bp) 4595
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Vector typeMammalian Expression ; membrane targeted HRP (mHRP)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namessHRP-TM
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Alt namemHRP
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Insert Size (bp)1206
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Mutationincludes signal sequence and a transmembrane domain before and after the cDNA for HRP
- Promoter CMV promoter including immediate early promoter/enhancer
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid expresses EGFP and HRP under the control of two CMV promoters. The membrane-targeted HRP construct (mHRP) was created by adding a signal sequence and transmembrane domain from human CD2 before and after the cDNA of HRP. Please see the associated publication for more information on this plasmid.
DNA (Catalog # 31156-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 31156-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-EGFP-ssHRP-TM was a gift from Hollis Cline (Addgene plasmid # 31156 ; http://n2t.net/addgene:31156 ; RRID:Addgene_31156) -
For your References section:
Membrane targeted horseradish peroxidase as a marker for correlative fluorescence and electron microscopy studies. Li J, Wang Y, Chiu SL, Cline HT. Front Neural Circuits. 2010 Feb 26;4:6. doi: 10.3389/neuro.04.006.2010. eCollection 2010. 10.3389/neuro.04.006.2010 PubMed 20204144
Map uploaded by the depositor.
