pET15-MTFmt
(Plasmid
#31174)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31174 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET15b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5400
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsE. coli DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMature form bovine mitochondrial methionyl-tRNA transformylase
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Alt nameMTFmt
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SpeciesB. taurus (bovine)
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Insert Size (bp)1071
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Entrez GeneMTFMT (a.k.a. BOS_10149)
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Tags
/ Fusion Proteins
- His (N terminal on backbone)
- thrombin cleavage (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET15-MTFmt was a gift from Linda Spremulli (Addgene plasmid # 31174 ; http://n2t.net/addgene:31174 ; RRID:Addgene_31174) -
For your References section:
Mammalian mitochondrial methionyl-tRNA transformylase from bovine liver. Purification, characterization, and gene structure. Takeuchi N, Kawakami M, Omori A, Ueda T, Spremulli LL, Watanabe K. J Biol Chem. 1998 Jun 12. 273(24):15085-90. 10.1074/jbc.273.24.15085 PubMed 9614118