pET21-MRPS2
(Plasmid #31342)

• Depositing Lab: Linda Spremulli
• Publication: Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Backbone

• Vector backbone: pET21
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression
• Selectable markers: CAM

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: In Bl21(De3) RIL conferring chloramphenicol resistance
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mitochondrial ribosomal protein S2 mature form
• Alt name: MRPS2
• Species: H. sapiens (human)
• Insert Size (bp): 741
• Mutation: Encodes the predicted mature form for incorporation into ribosomes, Amino acids 49-296
• Entrez Gene: MRPS2 (a.k.a. S2mt, CGI-91, MRP-S2)
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (destroyed during cloning)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: vector

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry