pET21-MRPL13
(Plasmid #31344)

• Depositing Lab: Linda Spremulli
• Publication: Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Backbone

• Vector backbone: pET21
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression
• Selectable markers: chloramphenicol

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: E. coli BL21 (DE3) RIL has chloramphenicol resistance on RIL plasmid
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mitochondrial ribosomal protein L13 mature form
• Alt name: MRPL13
• Species: H. sapiens (human)
• Insert Size (bp): 414
• Mutation: Mature form after removal of the predicted mitochondrial import signal Amino acids 40-178
• Entrez Gene: MRPL13 (a.k.a. L13, L13A, L13mt, RPL13, RPML13)
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: vector

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ATCC MGC-33899
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry