pINS-Puro
(Plasmid #31389)

• Depositing Lab: Yegor Vassetzky
• Publication: Dmitriev et al BMC Res Notes. 2008 . 1():135.

Backbone

• Vector backbone: pUNI10, pACYC-184
• Backbone manufacturer: Stephen Elledge and Fermentas respectively
• Backbone size w/o insert (bp): 1869
• Vector type: Cre/Lox
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): BW23474
• Growth instructions: BW23474 (pir::116), LB 37°C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: pac
• Alt name: puromycin-N-acetyl transferase
• Species: Streptomyces alboniger
• Insert Size (bp): 1441

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Clontech, Carlsbad, CA, USA; Fermentas, Lithuania

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

3nt insert at position 2985 and 1nt mismatch at 2956 of full plasmid sequence. These mutations do not affect function.