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Addgene

TSG101
(Plasmid #31412)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31412 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pACT2
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 8100
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tumor susceptibility gene 101
  • Alt name
    VPS23
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    400
  • Entrez Gene
    TSG101 (a.k.a. TSG10, VPS23)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TSG101 was a gift from Rachel Klevit (Addgene plasmid # 31412 ; http://n2t.net/addgene:31412 ; RRID:Addgene_31412)
  • For your References section:

    E2-BRCA1 RING interactions dictate synthesis of mono- or specific polyubiquitin chain linkages. Christensen DE, Brzovic PS, Klevit RE. Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. 10.1038/nsmb1295 PubMed 17873885