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pTRETightBI-RY-7
(Plasmid #31466)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31466 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 31466-D50 50 μg of DNA in Tris buffer $495
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DNA 31466-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pTRETightBI
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 2850
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Top10
  • Growth instructions
    Grow TopTen cells at 30C instead of 37C to reduce risk of recombination at the SV40 pA sites.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eYFP and mCherry
  • Species
    fluorescent proteins
  • Insert Size (bp)
    740
  • Tag / Fusion Protein
    • NLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI, BamHI (not destroyed)
  • 3′ cloning site NdeI, ClaI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

7 tandem miR-20 binding sites in mCherry 3'UTR

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRETightBI-RY-7 was a gift from Phil Sharp (Addgene plasmid # 31466 ; http://n2t.net/addgene:31466 ; RRID:Addgene_31466)

  • For your References section:

    MicroRNAs can generate thresholds in target gene expression. Mukherji S, Ebert MS, Zheng GX, Tsang JS, Sharp PA, van Oudenaarden A. Nat Genet. 2011 Aug 21;43(9):854-9. doi: 10.1038/ng.905. 10.1038/ng.905 PubMed 21857679
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