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(Plasmid #31604)


Item Catalog # Description Quantity Price (USD)
Plasmid 31604 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pEGFP-C1 (EGFP removed)
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 3941
  • Modifications to backbone
    Deleted EGFP (601-1390) from original pEGFP-C1 vector
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    E. coli DH5α, 16hrs at 37C
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    Kif5b (a.k.a. Khc, Khcs, Kns1, Ukhc)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer specific sequence primer
  • 3′ sequencing primer specific sequence primer
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    Kinesin-1B cDNA (GenBank accession BC090841, I.M.A.G.E. clone:30543821, MGC:102147) was obtained from Open Biosystems (Huntsville, AL)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Because the insert may be toxic to the bacterial strain, we recommend pooling and concentrating DNA preps.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKin1B was a gift from Anthony Brown (Addgene plasmid # 31604 ; ; RRID:Addgene_31604)
  • For your References section:

    Tight functional coupling of kinesin-1A and dynein motors in the bidirectional transport of neurofilaments. Uchida A, Alami NH, Brown A. Mol Biol Cell. 2009 Dec . 20(23):4997-5006. 10.1091/mbc.e09-04-0304 PubMed 19812246