-
Depositing Lab
-
Depositing OrganizationOhio State University
Located in United States of America -
Publication
-
Citations
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31604 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 31604-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 31604-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepEGFP-C1 (EGFP removed)
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3941
-
Modifications to backboneDeleted EGFP (601-1390) from original pEGFP-C1 vector
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Growth instructionsE. coli DH5α, 16hrs at 37C
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert namekinesin-1B
-
Alt nameKIF5B
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)3332
-
GenBank IDBC090841
-
Entrez GeneKif5b (a.k.a. Khc, Khcs, Kns1, Ukhc)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer specific sequence primer
- 3′ sequencing primer specific sequence primer
- (Common Sequencing Primers)
Resource Information
-
Addgene Notes
-
A portion of this plasmid was derived from a plasmid made byKinesin-1B cDNA (GenBank accession BC090841, I.M.A.G.E. clone:30543821, MGC:102147) was obtained from Open Biosystems (Huntsville, AL)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Because the insert may be toxic to the bacterial strain, we recommend pooling and concentrating DNA preps.
DNA (Catalog # 31604-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 31604-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pKin1B was a gift from Anthony Brown (Addgene plasmid # 31604 ; http://n2t.net/addgene:31604 ; RRID:Addgene_31604) -
For your References section:
Tight functional coupling of kinesin-1A and dynein motors in the bidirectional transport of neurofilaments. Uchida A, Alami NH, Brown A. Mol Biol Cell. 2009 Dec . 20(23):4997-5006. 10.1091/mbc.e09-04-0304 PubMed 19812246
