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miR-499
(Plasmid #31641)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31641 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pCDH-CMV-MCS-EF1
  • Backbone manufacturer
    System Biosciences
  • Backbone size w/o insert (bp) 7545
  • Vector type
    Mammalian Expression, Lentiviral ; miRNA expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miR-499
  • Alt name
    microRNA-499
  • Species
    H. sapiens (human)
  • Entrez Gene
    MIR499A (a.k.a. MIR499, MIRN499, hsa-mir-499a, mir-499a)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    miR-499 was a gift from Joseph Wu (Addgene plasmid # 31641)

  • For your References section:

    Dynamic microRNA expression programs during cardiac differentiation of human embryonic stem cells: role for miR-499. Wilson KD, Hu S, Venkatasubrahmanyam S, Fu JD, Sun N, Abilez OJ, Baugh JJ, Jia F, Ghosh Z, Li RA, Butte AJ, Wu JC. Circ Cardiovasc Genet. 2010 Oct 1. 3(5):426-35. 10.1161/CIRCGENETICS.109.934281 PubMed 20733065
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