Rabbit NHERF-1
(Plasmid
#31644)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31644 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBluescript SK-
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesolute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
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Alt nameNHERF
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SpeciesO. cuniculus (rabbit)
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Insert Size (bp)1895
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Mutationnone
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GenBank IDu19815
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Entrez GeneSLC9A3R1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer M13 T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rabbit NHERF-1 was a gift from Edward Weinman (Addgene plasmid # 31644 ; http://n2t.net/addgene:31644 ; RRID:Addgene_31644) -
For your References section:
Characterization of a protein cofactor that mediates protein kinase A regulation of the renal brush border membrane Na(+)-H+ exchanger. Weinman EJ, Steplock D, Wang Y, Shenolikar S. J Clin Invest. 1995 May . 95(5):2143-9. 10.1172/JCI117903 PubMed 7738182