Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31748||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Growth in Bacteria
SpeciesD. melanogaster (fly)
MutationContains only aa 1-432
Entrez GeneKhc-73 (a.k.a. Dmel_CG8183, CG8183, DmKIN73, DmKin73, DmKlp73, Dmel\CG8183, KHC-73, KIF 13A, KIF13A/B, KLP73, Khc73, Kin73, Kinesin-73, anon-EST:fe3C9, khc-73)
- Promoter Metallothionein
/ Fusion Protein
- GFP (C terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer MT1-F (Common Sequencing Primers)
Terms and Licenses
The construct contains V308M and H383R mutations in Khc73 relative to GenBank reference sequences and is as used in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Khc73(1-432)-GFP was a gift from Ron Vale (Addgene plasmid # 31748 ; http://n2t.net/addgene:31748 ; RRID:Addgene_31748)
For your References section:Kinesin-73 is a processive motor that localizes to Rab5-containing organelles. Huckaba TM, Gennerich A, Wilhelm JE, Chishti AH, Vale RD. J Biol Chem. 2011 Mar 4;286(9):7457-67. Epub 2010 Dec 18. 10.1074/jbc.M110.167023 PubMed 21169635