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pJRT.Agro.CLCrVA.010
(Plasmid #31810)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31810 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 31810-D50 50 μg of DNA in Tris buffer $495
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DNA 31810-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCAMBIA 1300
  • Backbone manufacturer
    Cambia Labs
  • Backbone size w/o insert (bp) 8935
  • Total vector size (bp) 12124
  • Modifications to backbone
    CLCrV VIGS A component containing a 711-bp full length smRSGFP gene was inserted between the SacI and SalI sites in the pCAMBIA 1300 backbone.
  • Vector type
    Agrobacterium Binary
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    JRTCLCrVA.010
  • Alt name
    JRT.010
  • Alt name
    CLCrVA:GFP
  • Species
    Begomovirus
  • Insert Size (bp)
    3189
  • GenBank ID
    EU541443.1 U70496

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer AACAGCTATGACCATG
  • 3′ sequencing primer GTAAAACGACGGCCAGT
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJRT.Agro.CLCrVA.010 was a gift from Niki Robertson (Addgene plasmid # 31810 ; http://n2t.net/addgene:31810 ; RRID:Addgene_31810)

  • For your References section:

    Method: low-cost delivery of the cotton leaf crumple virus-induced gene silencing system. Tuttle JR, Haigler CH, Robertson D. Plant Methods. 2012 Aug 1;8(1):27. doi: 10.1186/1746-4811-8-27. 10.1186/1746-4811-8-27 PubMed 22853641
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