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pBabe hygro 3xFLAG Dna2 dm (nuc + hel)
(Plasmid #31958)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31958 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe hygro 3xFLAG
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hDna2
  • Alt name
    Dna2L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3200
  • Mutation
    Changed start ATG to AAA, and D294A and K671E
  • GenBank ID
    NM_001080449
  • Entrez Gene
    DNA2 (a.k.a. DNA2L, hDNA2)
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PAC1 (not destroyed)
  • 3′ cloning site SAL1 (not destroyed)
  • 5′ sequencing primer pBabe 5'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

to get 3xFLAG Dna2 out cut with BAMH1 SAL1;
this vector expresses hDna2 ATPase/helicase and nuclease deficient.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe hygro 3xFLAG Dna2 dm (nuc + hel) was a gift from Sheila Stewart (Addgene plasmid # 31958 ; http://n2t.net/addgene:31958 ; RRID:Addgene_31958)
  • For your References section:

    Okazaki Fragment Processing-independent Role for Human Dna2 Enzyme during DNA Replication. Duxin JP, Moore HR, Sidorova J, Karanja K, Honaker Y, Dao B, Piwnica-Worms H, Campbell JL, Monnat RJ Jr, Stewart SA. J Biol Chem. 2012 Jun 22;287(26):21980-91. Epub 2012 May 7. 10.1074/jbc.M112.359018 PubMed 22570476