pRK5 FLAG metap2
(Plasmid #32004)

• Depositing Lab: David Sabatini
• Publication: Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Backbone

• Vector backbone: pRK5
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: metap2
• Entrez Gene: Metap2 (a.k.a. 4930584B20Rik, A930035J23Rik, AI047573, AL024412, AU014659, Amp2, MGC102452, Mnpep, p67, p67eIF2)
• Promoter: CMV, SP6
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note from the depositor: "metap2 is a control protein, we know that the metap2-containing constructs detect a ~60kd flag tagged protein by western blot, confirming that it actually expresses metap2 has not been relevant for our experiments"

Please note that Addgene's quality control sequence suggests that amino acid 51 (alanine) is missing from the metap2