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Addgene

pRK5 FLAG metap2
(Plasmid #32004)

Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 32004 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRK5
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    metap2
  • Entrez Gene
    Metap2 (a.k.a. 4930584B20Rik, A930035J23Rik, AI047573, AL024412, AU014659, Amp2, MGC102452, Mnpep, p67, p67eIF2)
  • Promoter CMV, SP6
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note from the depositor: "metap2 is a control protein, we know that the metap2-containing constructs detect a ~60kd flag tagged protein by western blot, confirming that it actually expresses metap2 has not been relevant for our experiments"

Please note that Addgene's quality control sequence suggests that amino acid 51 (alanine) is missing from the metap2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5 FLAG metap2 was a gift from David Sabatini (Addgene plasmid # 32004 ; http://n2t.net/addgene:32004 ; RRID:Addgene_32004)
  • For your References section:

    mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM. Cell. 2011 Aug 5;146(3):408-20. 10.1016/j.cell.2011.06.034 PubMed 21816276