Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pRK5 FLAG 17xS/T->A, catalytic dead lipin 1
(Plasmid #32008)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32008 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lipin1
  • Alt name
    fld; Lpin1; Kiaa0188; mKIAA0188; 4631420P06
  • Species
    M. musculus (mouse)
  • Mutation
    D712E, D714E AND S106A, S150A, (S281A, T282A), S285A, S287A, S293A, T298A, S328A, (S353A, S356A), S392A, S468A, S472A, S483A, S634A, S635A, (S647A, S648A)
  • GenBank ID
    NM_001130412
  • Entrez Gene
    Lpin1 (a.k.a. Lipin1, fld)
  • Promoter CMV, SP6
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The 17xS/T→ A mutant encompasses the following residues: S106, S150, (S281, T282), S285, S287, S293, T298, S328, (S353, S356), S392, S468, S472, S483, S634, S635, (S647, S648). Residues in parentheses were both mutated to alanine because the singly phosphorylated site in each case could not be unambiguously determined by mass spectrometry

S921A, S923A were previously reported to have been present in this plasmid, but these residues are in fact WT. This change should no affect the reported function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5 FLAG 17xS/T->A, catalytic dead lipin 1 was a gift from David Sabatini (Addgene plasmid # 32008 ; http://n2t.net/addgene:32008 ; RRID:Addgene_32008)
  • For your References section:

    mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM. Cell. 2011 Aug 5;146(3):408-20. 10.1016/j.cell.2011.06.034 PubMed 21816276