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pRK5 FLAG 17xS/T->A, catalytic dead lipin 1
(Plasmid #32008)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 32008 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    fld; Lpin1; Kiaa0188; mKIAA0188; 4631420P06
  • Species
    M. musculus (mouse)
  • Mutation
    D712E, D714E AND S106A, S150A, (S281A, T282A), S285A, S287A, S293A, T298A, S328A, (S353A, S356A), S392A, S468A, S472A, S483A, S634A, S635A, (S647A, S648A)
  • GenBank ID
  • Entrez Gene
    Lpin1 (a.k.a. Lipin1, fld)
  • Promoter CMV, SP6
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The 17xS/T→ A mutant encompasses the following residues: S106, S150, (S281, T282), S285, S287, S293, T298, S328, (S353, S356), S392, S468, S472, S483, S634, S635, (S647, S648). Residues in parentheses were both mutated to alanine because the singly phosphorylated site in each case could not be unambiguously determined by mass spectrometry

S921A, S923A were previously reported to have been present in this plasmid, but these residues are in fact WT. This change should no affect the reported function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5 FLAG 17xS/T->A, catalytic dead lipin 1 was a gift from David Sabatini (Addgene plasmid # 32008 ; ; RRID:Addgene_32008)
  • For your References section:

    mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM. Cell. 2011 Aug 5;146(3):408-20. 10.1016/j.cell.2011.06.034 PubMed 21816276