LPH2781 - Ub-K63R
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32160||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerMike Ellison - University of Alberta
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer CUP1 forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
BamHI-KpnI fragment from YEp96 (D. Ecker, U PENN) was cloned into pES7 (Mike Ellison, University of Alberta) cut with BamHI/KpnI.
K63R mutation introduced by quickchange of LHP308 (Addgene plasmid 32151).
2 µm S. cerevisiae origin
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LPH2781 - Ub-K63R was a gift from Linda Hicke (Addgene plasmid # 32160 ; http://n2t.net/addgene:32160 ; RRID:Addgene_32160)