pEGFP-N1-CaMKKbeta
(Plasmid #32453)

• Depositing Lab: Anthony Means
• Publication: Green et al J Biol Chem. 2011 Aug 12;286(32):28066-79. Epub 2011 Jun 13.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Modifications to backbone: FLAG tag added into NheI site by cutting and inserting linker with FLAG tag and NheI overhangs. Second NheI site removed by site-directed mutagenesis.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Calcium/Calmodulin dependent protein kinase kinase beta
• Alt name: CaMKKbeta
• Alt name: CaMKK2
• Species: M. musculus (mouse)
• GenBank ID: NM_145358.1
• Entrez Gene: Camkk2 (a.k.a. 6330570N16Rik, AW061083, mKIAA0787)
• Promoter: CMV
• Tags / Fusion Proteins:
  • GFP (C terminal on backbone)
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CMV

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A W374C mutation is also present in the pEGFP-N1-CaMKKbeta constructs that were used in this publication.