pOZ-FH-C-puro
(Plasmid #32516)

• Purpose: (Empty Backbone)
• Depositing Lab: Andrew Lassar
• Publication: Kumar et al Mol Biol Cell. 2009 Jul;20(14):3170-7. Epub 2009 May 20.

Backbone

• Vector backbone: pOZ-FH-C
• Backbone manufacturer: Created by Yoshihiro Nakatani
• Backbone size (bp): 7900
• Modifications to backbone: pOZ-FH-C-puro vector was made by modifying the pOZ-FH-C vector (Nakatani and Ogryzko, 2003). A PCR-amplified puromycin resistance gene (isolated from pBabe-puro) was cloned in place of interleukin-2Rα using NcoI and BamHI site.
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin
• Tag / Fusion Protein:
  • FLAG and HA (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Resource Information

• Supplemental Documents:
  • MCS
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pOZ-FH-C-puro vector was made by modifying the pOZ-FH-C vector (Nakatani and Ogryzko, 2003). A PCR-amplified puromycin resistance gene (isolated from pBabe-puro) was cloned in place of interleukin-2Rα using NcoI and BamHI site.