pcDNA3-Ha-Brca1 S1423A/S1524A
(Plasmid
#32539)
-
Depositing Lab
-
Depositing OrganizationSt. Jude Children's Research Hospital
Located in United States of America -
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32539 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 32539-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 32539-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepcDNA3
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameBRCA1
-
Alt nameBRCC1
-
Alt namePNCA4
-
Alt nameRNF53
-
SpeciesH. sapiens (human)
-
Insert Size (bp)5592
-
MutationS1423A/S1524A
-
GenBank ID672
-
Entrez GeneBRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
- Promoter CMV/rabbit B-Globin
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BAMH1 (not destroyed)
- 3′ cloning site XHO1 (not destroyed)
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made bypcDNA3 HA BRCA1 wild type provided by David Livingston, double mutant made in the Kastan lab at St. Jude Children's Research Hospital
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 32539-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 32539-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3-Ha-Brca1 S1423A/S1524A was a gift from Michael Kastan (Addgene plasmid # 32539 ; http://n2t.net/addgene:32539 ; RRID:Addgene_32539) -
For your References section:
Phosphorylation of serine 1387 in Brca1 is specifically required for the Atm-mediated S-phase checkpoint after ionizing irradiation. Xu B, O'Donnell AH, Kim ST, Kastan MB. Cancer Res. 2002 Aug 15;62(16):4588-91. PubMed 12183412