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pDEST 3x Flag-pcDNA5-FRT/T0-BRCA1
(Plasmid #52504)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52504 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEST 3' Triple Flag pcDNA5 FRT TO
  • Backbone size w/o insert (bp) 6856
  • Total vector size (bp) 8720
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin, Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BRCA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1864
  • Mutation
    siRNA resistant sequence: AGTATAATC
  • Entrez Gene
    BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST 3x Flag-pcDNA5-FRT/T0-BRCA1 was a gift from Daniel Durocher (Addgene plasmid # 52504 ; http://n2t.net/addgene:52504 ; RRID:Addgene_52504)
  • For your References section:

    A cell cycle-dependent regulatory circuit composed of 53BP1-RIF1 and BRCA1-CtIP controls DNA repair pathway choice. Escribano-Diaz C, Orthwein A, Fradet-Turcotte A, Xing M, Young JT, Tkac J, Cook MA, Rosebrock AP, Munro M, Canny MD, Xu D, Durocher D. Mol Cell. 2013 Mar 7;49(5):872-83. doi: 10.1016/j.molcel.2013.01.001. Epub 2013 Jan 17. 10.1016/j.molcel.2013.01.001 PubMed 23333306