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pcDNA5-FRT/T0-Flag-53BP1
(Plasmid #52507)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52507 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pcDNA5 FRT/T0-Flag
  • Backbone size w/o insert (bp) 5198
  • Total vector size (bp) 11114
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    53BP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5916
  • Mutation
    V128A and siRNA resistant sequence: ACGCGCTGACGACCCGCTT
  • Entrez Gene
    TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
  • Promoter CMV/TO
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-FRT/T0-Flag-53BP1 was a gift from Daniel Durocher (Addgene plasmid # 52507 ; http://n2t.net/addgene:52507 ; RRID:Addgene_52507)
  • For your References section:

    A cell cycle-dependent regulatory circuit composed of 53BP1-RIF1 and BRCA1-CtIP controls DNA repair pathway choice. Escribano-Diaz C, Orthwein A, Fradet-Turcotte A, Xing M, Young JT, Tkac J, Cook MA, Rosebrock AP, Munro M, Canny MD, Xu D, Durocher D. Mol Cell. 2013 Mar 7;49(5):872-83. doi: 10.1016/j.molcel.2013.01.001. Epub 2013 Jan 17. 10.1016/j.molcel.2013.01.001 PubMed 23333306