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pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A
(Plasmid #52505)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 52505 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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  • Vector backbone
    pDEST 3' Triple Flag pcDNA5 FRT TO
  • Backbone size w/o insert (bp) 6856
  • Total vector size (bp) 12772
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin, Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    53BP1 28A
  • Alt name
    siRNA resistant and ATM site-deficient construct of 53BP1
  • Species
    H. sapiens (human)
  • Mutation
    All 28 SQ/TQ motifs in N-term of 53BP1 have been mutated to Ala; siRNA resistant mutation in 53BP1 is ACGCGCTGACGACCCGCTT
  • Entrez Gene
    TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A was a gift from Daniel Durocher (Addgene plasmid # 52505 ; ; RRID:Addgene_52505)
  • For your References section:

    A cell cycle-dependent regulatory circuit composed of 53BP1-RIF1 and BRCA1-CtIP controls DNA repair pathway choice. Escribano-Diaz C, Orthwein A, Fradet-Turcotte A, Xing M, Young JT, Tkac J, Cook MA, Rosebrock AP, Munro M, Canny MD, Xu D, Durocher D. Mol Cell. 2013 Mar 7;49(5):872-83. doi: 10.1016/j.molcel.2013.01.001. Epub 2013 Jan 17. 10.1016/j.molcel.2013.01.001 PubMed 23333306