pCAG:mRFP1
(Plasmid
#32600)
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Depositing Lab
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Depositing OrganizationMemorial Sloan-Kettering Cancer Center
Located in United States of America -
Publication
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Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32600 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 32600-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 32600-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCAGGS
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemRFP1
- Promoter pCAGGs
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer 5'RFP (5'-cgtagaattcgccaccaatggctagcatgactgg)
- 3′ sequencing primer 3'RFP (5'-gcacgaattcgggcgccggtggagtggcggcc)
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The mRFP1 coding sequence was amplified by PCR using
primers 5'RFP (5'-cgtagaattcgccaccaatggctagcatgactgg) and
3'RFP (5'-gcacgaattcgggcgccggtggagtggcggcc) using Pfx
Polymerase (Invitrogen). The resulting product was
cloned into the EcoRI site of pCAGGS to generate pCXmRFP1. Transient transfection of Cos-7 cells using Fugene
6 Transfection Reagent as per manufacturer's recommendations (Roche) was used to evaluate pCX-mRFP1, and
verify that it produced robust red fluorescence
More Information: https://www.ncbi.nlm.nih.gov/pubmed/12060735 A monomeric red fluorescent protein. Campbell et al (PNAS. 2002 Jun 11; 99:12)
DNA (Catalog # 32600-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 32600-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG:mRFP1 was a gift from Anna-Katerina Hadjantonakis (Addgene plasmid # 32600 ; http://n2t.net/addgene:32600 ; RRID:Addgene_32600) -
For your References section:
Genetic and spectrally distinct in vivo imaging: embryonic stem cells and mice with widespread expression of a monomeric red fluorescent protein. Long JZ, Lackan CS, Hadjantonakis AK. BMC Biotechnol. 2005 Jul 4;5:20. 10.1186/1472-6750-5-20 PubMed 15996270
Map uploaded by the depositor.
