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Trip-GFP-hOCLN-ΔN
(Plasmid #32792)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32792 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTrip
  • Backbone manufacturer
    Gungxia Gao
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OCLN
  • Species
    H. sapiens (human)
  • Mutation
    Deletion of aa 1-63
  • Entrez Gene
    OCLN (a.k.a. BLCPMG, PPP1R115, PTORCH1)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Trip-GFP-hOCLN-ΔN was a gift from Tianyi Wang (Addgene plasmid # 32792 ; http://n2t.net/addgene:32792 ; RRID:Addgene_32792)
  • For your References section:

    The second extracellular loop dictates Occludin-mediated HCV entry. Liu S, Kuo W, Yang W, Liu W, Gibson GA, Dorko K, Watkins SC, Strom SC, Wang T. Virology. 2010 Nov 10;407(1):160-70. Epub 2010 Sep 6. 10.1016/j.virol.2010.08.009 PubMed 20822789