pET30 NHERF1
(Plasmid #32854)

• Depositing Lab: Edward Weinman
• Publication: Weinman et al J Clin Invest. 1998 May 15;101(10):2199-206.

Backbone

• Vector backbone: pet30a+
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5422
• Modifications to backbone: none
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
• Alt name: NHERF
• Species: O. cuniculus (rabbit)
• Insert Size (bp): 1200
• Mutation: none
• GenBank ID: U19815
• Entrez Gene: SLC9A3R1
• Promoter: T7
• Tags / Fusion Proteins:
  • his (N terminal on insert)
  • S16 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: V=EcoRV I =BssH1 (destroyed during cloning)
• 3′ cloning site: V=EcoRV i=EcoR1 (destroyed during cloning)
• 5′ sequencing primer: T7 promoter primer
• 3′ sequencing primer: T7 terminator primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pET 30a vector cut with EcoRV
NHERF from SK-cut with BSSH II and ECoRI.
Both filled in and blunt ligated.

insert at bp 5217-6918
aa 1-358 BP#5076 ATG
leader protein = 49 +9 from cDNA not usually translated
EK cuts 43 AA off sequence
nherf tranlates 5244