Mouse NHERF-1
(Plasmid #32950)

• Depositing Lab: Edward Weinman
• Publication: Weinman et al Biochim Biophys Acta. 1999 Oct 6;1447(1):71-6.

Backbone

• Vector backbone: pBluescript SK1
• Backbone manufacturer: stratagene
• Backbone size w/o insert (bp): 2960
• Modifications to backbone: none
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Slc9a3R1
• Alt name: NHERF
• Species: M. musculus (mouse)
• Insert Size (bp): 1065
• Mutation: none
• GenBank ID: U74079
• Entrez Gene: Nherf1 (a.k.a. EBP-50, NHE-RF, NHERF-1, Slc9a3r1)
• Promoter: t3
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: t3 primer
• 3′ sequencing primer: t7 primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

original clone from Stratagene's Mouse kidney cDNA library "UniZap" XR vector Cat # 93715