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Mouse NHERF-1
(Plasmid #32950)


Item Catalog # Description Quantity Price (USD)
Plasmid 32950 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pBluescript SK1
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2960
  • Modifications to backbone
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Copy number


  • Gene/Insert name
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
  • GenBank ID
  • Entrez Gene
    Slc9a3r1 (a.k.a. EBP-50, NHE-RF, NHERF-1, NHERF1)
  • Promoter t3
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer t3 primer
  • 3′ sequencing primer t7 primer
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

original clone from Stratagene's Mouse kidney cDNA library "UniZap" XR vector Cat # 93715

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse NHERF-1 was a gift from Edward Weinman (Addgene plasmid # 32950 ; ; RRID:Addgene_32950)
  • For your References section:

    Molecular cloning of the cDNA and promoter sequences for the mouse sodium-hydrogen exchanger regulatory factor. Weinman EJ, Steplock D, Zhang X, Akhter S, Shenolikar S. Biochim Biophys Acta. 1999 Oct 6;1447(1):71-6. 10.1016/S0167-4781(99)00100-1 PubMed 10500246