Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24421||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2265
Entrez GenePODXL2 (a.k.a. PODLX2)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
Full length PCLP-2 clone isolated from Origens fetal Brain Library clone #G11H9-11b
A S207F mutation in the gene has no known effect on function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PCLP-2/pCMV (100) was a gift from Steven Rosen (Addgene plasmid # 24421 ; http://n2t.net/addgene:24421 ; RRID:Addgene_24421)
For your References section:Identification of endoglycan, a member of the CD34/podocalyxin family of sialomucins. Sassetti C, Van Zante A, Rosen SD. J Biol Chem. 2000 Mar 24. 275(12):9001-10. 10.1074/jbc.275.12.9001 PubMed 10722749