pcDNA3.1_MMP-9-HA
(Plasmid #121172)

• Purpose: Expression of mouse MMP9
• Depositing Labs: M. Elizabeth Fini, Shinwu Jeong
• Publication: Jeong et al Am J Pathol. 2012 May;180(5):2028-39. doi: 10.1016/j.ajpath.2012.01.025. Epub 2012 Mar 20.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MMP9 (matrix metallopeptidase 9)
• Species: M. musculus (mouse)
• Insert Size (bp): 3100
• Mutation: See depositor comments below
• Entrez Gene: Mmp9 (a.k.a. B/MMP9, Clg4b, Gel B, MMP-9, pro-MMP-9)
• Promoter: CMV immediate/early
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains a F141L mutation in CLG4B (MMP9). This mutation is not known to affect plasmid function.