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pcDNA3-EGFP-Cdc42-T17N
(Plasmid #12976)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12976 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3-EGFP
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cdc42 DN
  • Alt name
    Cdc42
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    576
  • Mutation
    T17N Dominant negative
  • GenBank ID
    M57298
  • Entrez Gene
    CDC42 (a.k.a. CDC42Hs, G25K, TKS)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-EGFP-Cdc42-T17N was a gift from Gary Bokoch (Addgene plasmid # 12976 ; http://n2t.net/addgene:12976 ; RRID:Addgene_12976)
  • For your References section:

    Rho family proteins modulate rapid apoptosis induced by cytotoxic T lymphocytes and Fas. Subauste MC, Von Herrath M, Benard V, Chamberlain CE, Chuang TH, Chu K, Bokoch GM, Hahn KM. J Biol Chem. 2000 Mar 31. 275(13):9725-33. 10.1074/jbc.275.13.9725 PubMed 10734125